In Paris Nathalie Manfrino enters L’Ecole Normale de Musique de Parisin 1995. In June 2000, she graduates and is nominated 'first with special congratulations' from the Jury. Since her graduation, she has won various competions and prizes such as the 'E.Chausson' at the Maîtres du Chant Français competition, the International Vocal Competition of Marmande, the 'Grand Prix' at the International Vocal Competition of Toulouse. Nathalie Manfrino was awarded 'Revelation of the Year', in the Lyrical Artist category in 2006.
She gave several concerts in duet with Rolando Villazon in the Caracalla's Termes in Rome and during the Trondheim Festival in Norway. She also sang with the Chamber Orchestra of Geneva, and in the Smetana Hall of Prague, in the Palau de les Arts of Valencia (Spain). She sings also in the Auditorium A. Kraus of Las Palmas de Gran Canaria, in the Acropolis of Nice, in the National Concert hall of Dublin than with 'La Philarmonie' au Luxembourg.
In 2009, she participated with pop singers such as Sting and Elvis Costelo to the recording of the new opera 'Welcome to the voice '.